![]() ![]() Moreover, differentiation of NSC often results in variable and heterogeneous cultures of neurons, glia and undifferentiated cells, which impedes many downstream applications requiring purified or defined cell populations, such as in vitro assays, transplantation and microarrays. ![]() Unfortunately, the robustness of these methods is hampered by batch-to-batch variability of isolated NSC. In principle, these cells can differentiate to neurons and glia, providing an endless supply of cells for in vitro and in vivo assays. NSC can be manually isolated and be propagated as monolayer cultures for many passages. There are several neural induction methods that enrich for NSC or neurons using spontaneous differentiation, chemical induction or mouse stromal feeder cells, ,,. However, developing well-defined conditions to generate pure populations of specific cell types is critical to achieve these goals. Thus, hESC and hiPSC differentiation offers a unique opportunity for therapy development, drug screening, disease modeling, and tissue replacement. Human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) have the ability to differentiate to somatic-like cells. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: JE, NE, RIP, JGV and CTC own stock in Becton, Dickinson and Company. CTC, NE, RP, JM, JE and JGV were supported by Becton Dickinson Biosciences. This work was partially supported by a grant from CIRM to LSBG (RC1-00116-1) and to MM (RC1-00131-1) and HHMI. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: SHY, RLN and MAI were partially supported by the CIRM Interdisciplinary Stem Cell Training Program at UCSD (grant T1-00003 Larry Goldstein, PD). ![]() ![]() Received: SeptemAccepted: FebruPublished: March 2, 2011Ĭopyright: © 2011 Yuan et al. PLoS ONE 6(3):Įditor: Martin Pera, University of Southern California, United States of America (2011) Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells. Purified glia were mitotic and could mature to GFAP-expressing astrocytes in vitro and in vivo.Ĭitation: Yuan SH, Martin J, Elia J, Flippin J, Paramban RI, Hefferan MP, et al. Purified neurons were viable, expressed mature and subtype-specific neuronal markers, and could fire action potentials. A population of neurons that was CD184 −/CD44 −/CD15 LOW/CD24 + and a population of glia that was CD184 +/CD44 + were subsequently purified from cultures of differentiating NSC. Sorted NSC could be propagated for many passages and could differentiate to mixed cultures of neurons and glia in vitro and in vivo. We isolated a population of NSC that was CD184 +/CD271 −/CD44 −/CD24 + from neural induction cultures of hESC and human induced pluripotent stem cells (hiPSC). From this analysis we identified prospective cell surface signatures for the isolation of NSC, glia and neurons. We performed an unbiased FACS- and image-based immunophenotyping analysis using 190 antibodies to cell surface markers on naïve human embryonic stem cells (hESC) and cell derivatives from neural differentiation cultures. ![]()
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